Identification of a novel interleukin-9 receptor complex in non-hematopoietic cells

Abstract Beyond cell-cell contact, communication at a distance requires the secretion of cytokines that bind to specific receptors on responsive cells. Interleukin (IL)-9 is cytokine with pleiotropic effects not only immune cells contribute progression tumor and autoimmunity, but also structural including airway intestinal epithelial involved in development inflammatory allergic diseases. Typically, IL-9 receptor (IL-9R) functions as complex two subunits – α-chain (IL-9Rα) common γ (γc) chain, shared other receptors. Although express IL-9Rα, they lack expression γc chain. Thus, it still unclear how transmits signal through single IL-9Rα chain non-hematopoietic This study aimed explore possibility like type II IL-4Rα, can pair IL-13Rα1 forming IL-9R mediate responses We demonstrated IL-9Rα/IL-13Rα1 was detected situin mouse tracheal human small (SAEC) using proximity ligation assay. STAT3 activated after exposure vitroin SAEC when co-cultured neutralizing antibody targeting activity returned its basal level presence anti-IL-13Rα1 antibody, providing further evidence Strikingly, asthma-associated genes are upregulated upon stimulation RNA-seq analysis. Collectively, our results identify putative - composed IL-13Rα1, mechanism for potential role asthma.